Recentdevelopmentsinqualitativeandquantitativeanalysisofphytochemicalconstituentsandtheirmetabolitesusingliquidchromatography-massspectrometry•HaifengWu弘R,•JianGuob-®,•ShilinChena®,•XinLiuc-日，•YanZhoud-°,•XiaopoZhang8-®,•XudongXua-■■®aInstituteofMedicinalPlantDevelopment,ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,Beijing100193,PRChinabDepartmentofDMPK,AstraZenecaPharmaceuticals,35GatehouseDrive,Waltham,MA02451,USAcBeijingEntry-ExitInspectionandQuarantineBureau,Beijing100026,PRChina«ChengduInstituteofBiology,ChineseAcademyofSciences,Chengdu610041,PRChinahttp://dx.doi.org/10.1016/jjpba.2012.09.004,HowtoCiteorLinkUsingDOIPermissions&ReprintsViewfulltextPurchase$31.50AbstractOverthepastfewyears,theapplicationsofliquidchromatographycoupledwithmassspectrometry(LC-MS)innaturalproductanalysishavebeendramaticallygrowingbecauseoftheincreasinglyimprovedseparationanddetectioncapabilitiesofLC-MSinstruments.Inparticular,novelhigh-resolutionhybridinstrumentslinkedtoultra-high-performanceLCandthehyphenationsofLC-MSwithotherseparationoranalyticaltechniquesgreatlyaidunequivocalidentificationandhighlysensitivequantificationofnaturalproductsattraceconcentrationsincomplexmatrices・Withtheaimofprovidinganup-to-dateoverviewofLC-MSapplicationsontheanalysisofplant-derivedcompounds,paperspublishedwithinthelatestyears(2007-2012)involvingqualitativeandquantitativeanalysisofphytochemicalconstituentsandtheirmetabolitesaresummarizedinthepresentreview.Afterbrieflydescribingthegeneralcharacteristicsofnaturalproductsanalysis,themostremarkablefeaturesofLC-MSandsamplepreparationtechniques,thepresentpapermainlyfocusesonscreeningandcharacterizationofphenols(includingflavonoids),alkaloids,terpenoids,steroids,coumarins,lignans,andmiscellaneouscompoundsinrespectiveherbsandbiologicalsamples,aswellastraditionalChinesemedicine(TCM)prescriptionsusingtandemmassspectrometer.ChemicalfingerprintinganalysisusingLC-MSisalsodescribed.Meanwhile,instrumentalpeculiaritiesandmethodologicaldetailsareaccentuated.Keywords•Liquidchromatography;•Massspectrometry;Medicinalplants;TraditionalChinesemedicine;•Fingerprinting抽象在过去的几年中，液相色谱与质谱联用（LC-MS）在天然产物分析的应用都得到了显着增长，因为日益提高的分离和检测能力的LC-MS仪器。特别是，新颖的高分辨率混合工具与其他分离或分析技术链接到超高性能LC的LC-MS和断字大大有助于在复杂基质中的微量浓度的明确识别和天然产品的高度敏感的定量。提供最新的植物性化合物的分析概述LC-MS应用的目的，论文发表在最近几年（2007-2012年），涉及的植物化学成分及其代谢物的定性和定量分析总结在本次审査中。在简要说明天然产物的分析，最显着的特点LC-MS和样品制备技术的一般特点，本论文主耍集中在酚类物质（包括类黄酮），生物碱，菇类化合物，类固醇，香克素，木脂素类化合物的筛选和鉴定，和杂项在各自的草药化合物和生物样品，以及中国传统医学（中医）处方使用串联质谱仪。化学指纹分析用LC-MS也被描述。同时，仪器的特点和方法的细节突出。Figuresandtablesfromthisarticle:Fig.1.Chemicalstructuresofcagedxanthones(1-15)identifiedfromG.hanburyiReproducedwithpermissionfrom[89].氧杂蔥酮的化学结构(1-15)确定Fig.2.UHPLC-MSchromatogramsofthestandardxanthonesandthecrudeextractsofG.hanburyi(A)UHPLCchromatogramsof12standardxanthones,(B)TICofthe12standardxanthones,(C)UHPLCchromatogramsofthecrudeextractsofG.hanburyi.(D)TICofthecrudeextractsofG.hanburyi.o2oUHPLC-MS色谱图的标准叱吨酮和G.hanburyi粗提物中。(A...