AffecthemagglutinationFactorsAnalysisoftestresultsAuthor:Ting-tingonXiumeiWangNana[Abstract]Objective:Toanalyzethetestresultsaffectthehemagglutinationtestfactors.Methods:4447casesofoutpatientandinpatienttrackingobservation,inspectionanditsrelatedindexeswere:hemagglutinationtestresultsaffectthemainfactorsofdisease,drugsandsamplesthreeaspectsConclusion:Analysisofcoagulationtestresultsinconjunctionwiththepatient’sconditionandotherfactorsconsidered.[Keywords]hemagglutinationtest;prothrombintime;disease;drugs;sampleProthrombintime(PT)determinationoftheenzymeandactivatedpartialthromboplastintime(APTT),asreflectedinthedeterminationofthemechanismofbloodcoagulationscreeningtests,butalsoasascreeningtestforcoagulationabnormalitiesinthediagnosisofbleedingdisorders,preoperativeinspection,monitoringofanticoagulanttherapyhasanimportantrole.1Materialsandmethods1.1SampleSource:July23,2010toJuly22,2011inourhospitaloutpatientsandinpatients.1.2Instrumentsandreagents:CompactXGermanyBEautomaticcoagulationanalyzer,plugEllisreagent,ISI:1.04.Adaytodoqualitycontrolsamplebeforeyougo,donotgetoutofcontrolsample.ForeachPT↑(normalrange11~14s,↑indicatesincrease),APTT↑(normalrange23~43s)ofthespecimenshavebeenreviewed,havebeenexcludedinstruments,reagentsandtestpersonnelerrorscausedbyimproperoperation.1.3Samplecollectionandtesting:useofanticoagulantsforthe109mmol/Lsodiumcitrateanticoagulant1:9(ie0.2mLplus1.8mLofbloodanticoagulant),3000r/mincentrifugation10min,immediatelyonthetestmachine.2results2.1hospitalsubjectshemagglutinationtestresultsinTable1.ItcanbeseenfromTable1,inadditiontomedicalcenters,therestofthedepartmentshavePT,APTTincreasedphenomenontoICU,pediatric,oncologyHematology,themostobviousemergencydepartment.2.2factorofmanydiseaseshaveagreatimpactontheresults,suchashepatitisduetolivercelldamage,especiallyliverdamage,livercellsyntheticfibrinogen(FIB)blocked,theFIBdecreased,whilevitaminKdeficiencyorseveresynthesisofvariouscoagulationfactordeficienciesaffectthesynthesis,leadingtoavarietyofclottingfactordeficiency,thepatient’sbloodcoagulationinthelowstate,withableedingtendency,manifestedasthepatient’sPT,APTTvaryingdegreesofextension,FIBreducedtovaryingdegrees,andtheresultsaremorehealthysomedifference.disseminatedintravascularcoagulation(DIC),duetothelargeconsumptionofcoagulationfactorsandfibrindegradationproductsgenerated(FDP)theroleofthrombinantagonist,extendthePT,mainlyinpatientswithsurgicalICUTable1thevarioussectionshemagglutinationtestresultsstatistics[n(%)]2.3Factorsaffectingthehightestresults:(1)specimencollectionvolume(0.2mLanticoagulantplus1.8mLofblood,amountingto2.0mL)toreducetheimpactoftestresultsinTable2,lessblood,therelativeanticoagulantmoretimetoextendtheresultscausedetermination;thecontrarymorethanblood,arelativelylessanticoagulant,timemeasurementresults(2)transfusionofblooddilutedtoextendtheresultscausebloodclotting,bloodtransfusionre-sendingthesamplebeforeandafterthecoagulationtestresultswereTable3(3)samplesofspacetoplacethehigherthetemperature,thelongerthegreatertheimpactonthetestresults,PTisstableat4℃for24h,20℃lessthan6h,30℃lessthan4h;APTTin4℃lessthan6h,20℃lessthan4h,30℃lessthan2h[2].specimensatroomtemperaturefordifferenttimesofcoagulationresultsinTable4.2.4regardlessofthesizeoftheclotspecimenstherewillaffectthetestresults.Specimenplacedwithintime3hmeasuredinvitroresults...