健脾与补肾对脾虚模型大鼠学习记忆及脑内P物质、血管活性肠肽的影响【摘要】目的探讨脾虚模型大鼠学习记忆和脑内P物质(SP)、血管活性肠肽(VIP)的变化,以及健脾、补肾中药对上述变化的改善作用。方法采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,检测健脾与补肾对其学习记忆及脑内SP、VIP变化的影响。结果水迷宫实验学习期和记忆期,模型组游全程时间长,错误次数多；健脾组与补肾组游全程时间短,错误次数少。跳台实验学习期和记忆期,模型组错误次数较多,潜伏期短；健脾组错误次数较少,潜伏期长；在学习期补肾组错误次数较少,潜伏期较长,但记忆期错误次数较多。模型组SP免疫阳性反应物在下丘脑腹侧核、海马CA1区、前额叶皮层显著减少；健脾组在上述脑区则显著增加；补肾组海马CA1区和前额叶皮层明显增加,但在下丘脑腹侧核减少。模型组VIP免疫阳性反应物在海马CA1区、前额叶皮层显著减少；健脾组与补肾组海马CA1区、前额叶皮层VIP免疫阳性反应物显著增加。结论脾虚模型大鼠学习记忆及脑内对学习记忆有促进和增强作用的SP、VIP明显下降。健脾、补肾中药对上述变化有明显改善作用。【关键词】脾虚模型大鼠；学习记忆；P物质；血管活性肠肽Abstract：ObjectiveToinquireintothechangesoflearningandmemoryabilityandthelevelofSP,VIPinthebrainofthemodelofSpleeninsufficiency.Toobservetheinfluenceinvigoratingspleenandrestoringvirilityonabovechanges.MethodsEstablishingthespleeninsufficiencymodelofratbyadoptingbitterdeclinediarrhea,wildeatingandoverworking,toexamineinfluenceofinvigoratingspleenandrestoringvirilityontheabilityoflearningandmemorywiththechangeofSPandVIPinthebrain.ResultsIntheperiodofstudyandrememberofthewatermazeexperiment,theswimwholedistancetimewaslongandmistakenumberwasmanyinthemodelgroup；theswimwholedistancetimewasshortandthemistakenumberwaslittleinthegroupofinvigoratingtheSpleenandrestoringvirility.Intheperiodofstudyandrememberofthedivingplatformexperiment,themistakenumberofismoreandtheincubationperiodwasshortinthemodelgroup；themistakenumberwaslessandtheincubationperiodwaslonginSpleeninvigratinggroup.Attheperiodofremembermistakenumberwasmore.Inthemodelgroup,theimmunoreactionmasculinesubstanceofSPleveldecreaseobviouslyinthehypothalamusventralkernel,thehippocampiCA1areaandforehandcortex；onabovementionedparts,thegroupofinvigoratingspleenandrestoringvirilityincreasesdistinctly,inthegroupofrestoringvirilityincreasesdistinctlyinthehippocampiCA1areaandforeheadcortex,butdecreasedin---本文于网络，仅供参考，勿照抄，如有侵权请联系删除---hypothalamusventralkernel.Inthemodelgroup,theimmunoreactionmasculinesubstanceofVIPleveldecreasedobviously；inthehippocampiCA1areaandcortex；onabovementionedparts,thegroupsofinvigoratingspleenandrestoringvirilityincreasesobviously.ConclusionInthemodelgroup,theabilityoflearingandmemorywithSPandVIPlevelinthebrainchanged.Invigoratingspleenandrestoringvirilitycanimproveabovechangesremarkably.Keywords：modelofinsufficiencySpleen；learningandmemory；SP；VIP中医学认为,脾为后天之本,气血生化之源,藏意主思。肾为先天之本,主藏精,藏志。《灵枢本神》提出：“脾藏营,营舍意”,“肾藏精,精舍志”。脾与肾在神志活动中具有重要作用,而且脾与肾先天与后天相互资生,相互促进。本实验观察脾虚模型大鼠学习记忆和对学习记忆有促进和增强作用的P物质(SP)、血管活性肠肽(VIP)的变化,以及健脾、补肾中药对上述变化的改善作用。1材料和方法1.1实验动物及分组清洁级雄性Wistar大鼠,体重(160±10)g,北京维通利华实验动物技术有限公司提供。常规适应性饲养1周后,48只大鼠随机分成正常组、模型组、健脾组、补肾组,每组各12只。1.2实验药物配制造模药物由大黄、厚朴、枳实组成,按2...