龙血竭对急性心肌梗死家兔左心功能的保护研究【摘要】目的探?龙血竭对急性心肌梗死家兔左心功能的保护作用。方法将30只家兔随机分为三组，假手术组、心肌梗死组、龙血竭干预组，每组10只。采用左冠状动脉前降支结扎法，建立急性心肌梗死模型。观察HE染色的心肌组织结构变化，测量心率、左心室收缩末压(LVSP).左心室舒张末压(LVEDP)、左心室内压最大上升速率(+dp/dtmax)、左心室内压最大下降速率(-dp/dtmax)0结果龙血竭干预组心肌组织结构明显好于心肌梗死组；心肌梗死组和龙血竭干预组其心率较假手术组明显减慢，龙血竭干预组心率减慢更明显(P<0.05或0.01)。心肌梗死组、龙血竭干预组与假手术组比较，其LVSP均明显下降、LVEDP均明显升高，而心肌梗死组与龙血竭干预组比较，其LVSP下降更明显、LVEDP升高更明显(P〈0.05或0.01)。心肌梗死组和龙血竭干预组其+dp/dtmax和-dp/dtmax均较假手术组明显下降，且心肌梗死组较龙血竭干预组下降更明显，差异有统计学意义(P〈0.01)。结论龙血竭对急性心肌梗死家兔左心功能具有较好的保护作用。【关键词】龙血竭；急性心肌梗死；左心功能；保护中图分类号：R542.2+2文献标识码：ADOI:10.3969/j.issn.10031383.2017.01.004AstudyonprotectioneffectofRcsinaDraconisonleftventricularfunctionofacutemyocardialinfarctionrabbitsCHENGChuyongl,LIANGLimei2,LIUYan3,LIZhile3,LIKela3,LIUWenjingl,ZHANGZhuohual,HUANGZhaohe3A(1.GraduateSchool,2.DepartmentofLaboratoryMedicineofAffiliatedHospital,3.DepartmentofCardiologyofAffiliatedHospital,YoujiangMedicalUniversityforNationalities,Raise533000,China)[HJ1*2][HJ][Abstract]ObjectiveTostudyprotectioneffectofResinaDraconisonleftventricularfunctionofacutemyocardialinfarctionrabbits・Methods30rabbitswererandomlydividedintoshamoperationgroup,myocardialinfarctiongroupandResinaDraconisinterventiongroupwith10rabbitsineachgroup・Acutemyocardialinfarctionmodelwasestablishedbyleftanteriordescendingcoronaryarteryligationmethod.ThechangesofmyocardialtissuestructurewereobservedbyHEstaining,andheartrate,leftventricularendsystolicpressure(LVSP),leftventricularenddiastolicpressure(LVEDP),maximumriserateofleftventricularpressure(+dp/dtmax)andmaximumdescendingrateofleftventricularpressure(-dp/dtmax)weremeasured・ResultsThestructureofmyocardiumintheResinaDraconisintervontiongroupwassignificantlybetterthanthatinthemyocardialinfarctiongroup.HeartratesofmyocardialinfarctiongroupandResinaDraconisinterventiongroupsignificantlysloweddowncomparedwiththatoftheshamoperationgroup,andthatoftheResinaDraconisinterventiongroupdecreasedmoresignificantly(P表1三组家兔心率、LVSP、LVEDP、+dp/dtmax>-dp/dtmax(n=10,±s)分组心率(次/分)LVSP(mmHg)LVEDP(mmHg)+dp/dtmax(mmHg/ms)-dp/dtmax(mmllg/ms)假手术组291.90±28.25110.86±7.58-15.86土5.221.97±0.12-2.04±0.14心肌梗死组272.50±2.72a44.84±5.71B16.15±1.48b0.56±0.llb-0.60±0.08b龙血竭干预组240.50土4.53bc71.12±6.12bc-12.48±3.15acl.28±0.93bc-1.29±0.14bcF24.48259.68235.38418.71329.20P<0.001<0.001<0.001<0.001<0.001注：与假手术组比较，aP<0.05,bP<0.01；与心肌梗死组比较，cP<0.01o3讨论急性心肌梗死发生后，冠状动脉堵塞导致心肌细胞严重持久缺血缺氧，心肌细胞坏死，心肌纤维断裂溶解，从而出现明显的心功能不全，这是导致急性心肌梗死患者死亡的重要因素之一。另外，当急性心肌梗死发牛时，很多炎症因子如TNFQ、IL10、ICAM1等参与其病理生理过程，使心室重构，进一步加重心功能的恶化［2~3］o我们从本次实验心肌组织HE染色结果图中不难看出，假手术组家兔心肌组织结构清晰，肌节明显，染色浓淡均匀（如图2A）,而心肌梗死组心肌组织IIE染色中可见染色颜色明显加深，肌纤维水肿明显，排列结构明显紊乱，符合心肌梗死后心肌组织改变图像（如图2B）...