作物学报ACTAAGRONOMICASIN1CA2011,37⑸:811-819ISSN0496-3490;CODENTSHPA9DOI:10J724/SRJ.1006.201L00811利用SRAP标记分析我国甜菜三大产区骨干材料的遗传多样性王茂羊S吴则东123王华忠1，2"|黑龙江省普通高校甜菜遗传育种重点实验室/黑龙江大学.黑龙江哈尔滨150080;2中国农业科学院北方糖料作物资源与利用重点开放实验室，黑龙江哈尔滨150080;3中国农业科学院甜菜研究所/黑龙江大学农作物研究院，黑龙江哈尔滨150080摘要：利用SRAP分子标记，选用甜菜中SRAP的88对引物组合分别对4个经济性状差异较大的代表性品系(高产型、高产低糖抗丛根病型、标准型、中产高糖抗褐斑病型)进行扩增，筛选出有效引物组合33对。采用筛选的33对引物检测我国三大产区的241份甜菜材料及9份外国材料，扩增到719条带，其中多态性条带459条，多态性条带的比率平均为63.8%。利用MEGA3」软件中的Computeover-allmean计算，组内品种间平均遗传距离为0.4165,平均遗传相似系数为0.6593o遗传相似系数平均值为外国品种(0.7528)＞单胚品系(0.6945)＞多胚四倍体品系(0.6816)＞多胚二倍体品系(0.6612)。利用MEGA3.1软件，在遗传距离0.20处将供试材料分为4大类群。结果表明，我国三大产区供试材料遗传多样性丰富，其中东北产区优于华北与西北产区。利用POPGEN32软件将供试材料与外国品种分为两类，表明我国材料与外国品种的遗传基础存在较大差异。关键词：甜菜;SRAP标记；种质资源；遗传多样性GeneticDiversityofMajorSugarBeetVarietiesfromThreeRegionsofChinawithSRAPMarkersWANGMao-Qian1,2,3,WUZe-Dong1andWANGHua-Zhong11KeyLaboratoryofSugarBeetGeneticBreeding/HeilongjiangUniversity,Harbin150080.6ina;2KeyLaboratoryofNorthSugarCropResourceandUtilization,ChineseAcademyofAgriculturalSciences,Harbin150080,China;3SugarBeetResearchInstituteChineseAcademyofAgriculturalSc＞-ences/CropAcademyofHeilongjiangUniversity,Harbin150080,ChinaAbstract:Shortageofsugarbeetgermlasmresourcesresultsinthelaginresearchesofbreedingandmolecularbiology.Itisnece-ssarytoanalyzethemajorvarietiesfromthreemajorproductionregionsofGiina・Eighty-eightprimerpairswereusedtoamplifythegenomicDNAfromleavesoffourtypesofsugarbeetvarietieswithdifferenteconomictraits,whichcontainhighyieldtype;highyield,lowsugarandRhizomaniaresistanttype;standardtype;mediumyield,highsugarandanti-brownspottype,and33ofwhichwereobtainedtobewithavailability.Twohundredandforty-onevarietiesfromthreemajorregionsofChinaandnineva*rietiesfromabroadweredetectedwith33primerpairsofSRAPmarkers.Atotalof719unambiguousbandswereobtained,459ofwhichwerepolymorphic.Theaverageratioofpolymorphicbandswas63.8%・Computeover-allmeanshowedthatgeneticdis-tancewas0.4165,geneticsimilarityamongvarietieswas0.6593,thegeneticsimilaritieswere0.7528amongforeignvarieties,0.6945amongmonogermvarieties,0.6816amongpolygermtetrapioidvarieties,and0.6612amongpolygermdiploidvarieties・Atotalof250varietiesweredividedintofourclustergroupsbasedonclusteranalysisbyMEGA3.1(atinterceptof0.2).EachgenqxwlfromthreemajorregionsofsugarbeetproductioninChinashowedhighlevelofgeneticdiversity,ofwhichtheNorth-eastgenepoolshowedthehighest.ThevarietiesfromChinaandabroadwereclassifiedintotwodifferentgroupsbyPOPGEN32＞Thisindicateddefinitedifferenceinthegeneticbackgroundbetweenforeignandnativevarieties.Keywords:Sugarbeet;SRAPmarker;Germplasmresource;Geneticdiversity甜菜是我国重要的糖料作物，种植历史已有百传基础狭窄，而巳缺少分子水平的深入研究，致使年之久。但中国不是甜菜起源国，种质资源缺乏，遗育种水平提高缓慢。为培育出更高产、抗病、高糖本研究由国家“十一五”科技支撑卄划靈点项目(2006BAD01A06)资助。•通讯作^(Correspondingauthor):王华忠...