不同基原藁本药材的指纹图谱建立及其化学计量学分析梁慧潘晓君吕渭升杨文惠罗宇琴潘礼业霍文杰李振雨中圖分类号R284.1文献标志码A1001-0408（2021）19-2358-05DOI10.6039/j.issn.1001-0408.2021.19.09摘要目的：建立藁本、辽藁本、新疆藁本3种不同基原藁本药材的超高效液相色谱（UPLC）法指纹图谱，并进行化学计量学分析，为不同基原藁本药材的鉴别提供参考。方法：采用UPLC法检测并结合《中药色谱指纹图谱相似度评价系统（2012版）》建立23批不同基原藁本药材的指纹图谱，并进行色谱峰指认和相似度评价。采用聚类分析（CA）、主成分分析（PCA）及正交偏最小二乘判别分析（OPLS-DA）等化学计量学分析方法对不同基原藁本药材进行分析，筛选差异成分。结果：藁本、辽藁本、新疆藁本药材的UPLC指纹图谱中分别标定了13、11、11个特征峰，其中5号峰为阿魏酸;相似度评价结果显示，新疆藁本药材与辽藁本药材间的相似度为0.312～0.541，新疆藁本药材与藁本药材间的相似度为0.324～0.682，藁本药材与辽藁本药材间的相似度为0.312～0.671，表明不同基原藁本药材间的差异较大。经CA、PCA、OPLS-DA发现，不同基原藁本药材各自聚为一类;10号峰、13号峰、12号峰、7号峰、6号峰所代表的化学成分为这3种基原藁本药材的差异成分。结论：本研究建立了不同基原藁本药材的UPLC指纹图谱，筛选出了5种差异成分，可用于鉴别不同基原的藁本药材。关键词藁本;辽藁本;新疆藁本;基原鉴别;超高效液相色谱法;指纹图谱;聚类分析;主成分分析;正交偏最小二乘判别分析FingerprintEstablishmentofRhizomaLigusticifromDifferentOriginsandTheirChemometricsAnalysisLIANGHui，PANXiaojun，LYUWeisheng，YANGWenhui，LUOYuqin，PANLiye，HUOWen激e，LIZhenyu（GuangdongYifangPharmaceuticalCo.，Ltd./GuangdongProvincialKeyLaboratoryofTraditionalChineseMedicineFormulaGranule，GuangdongFoshan528244，China）ABSTRACTOBJECTIVE：ToestablishUPLCfingerprintofLigusticumsinense，LigusticumjeholenseandConioselinumvaginatium，andtoconducttheirchemometricsanalysissoastoprovidereferencefortheidentificationofRhizomaLigusticifromdifferentorigins.METHODS：UPLCmethodcombinedwithSimilarityEvaluationSystemofTCMChromatographicFingerprints（2012edition）wereusedtoestablishthefingerprintsofRhizomaLigusticifromdifferentorigins.Chromatographicpeakidentificationandsimilarityevaluationwerecarriedout.Clusteranalysis（CA），principalcomponentanalysis（PCA）andorthogonalpartialleastsquaresdiscriminantanalysis（OPLS-DA）wereperformedtoanalyzeRhizomaLigusticifromdifferentorigins，andscreendifferentialcomponents.RESULTS：Totally13，11，11characteristicpeakswereidentifiedinUPLCfingerprintsofL.sinense，L.jeholenseandC.vaginatium，respectively.SimilarityevaluationshowedthatthesimilaritybetweenC.vaginatiumandL.jeholensewere0.312-0.541;thatbetweenC.vaginatiumandL.sinensewere0.324-0.682;thatbetweenL.sinenseandL.jeholensewere0.312-0.671，indicatingtherewasgreatdifferenceamongRhizomaLigusticifromdifferentorigins.CA，PCAandOPLS-DAshowedthatRhizomaLigusticifromdifferentoriginswereclusteredintoeachcategoryrespectively;chemicalcomponentsrepresentedbypeak10，peak13，peak12，peak7andpeak6weredifferentialcomponentsforRhizomaLigusticifrom3origins.CONCLUSIONS：ThestudyestablishesUPLCfingerprintofRhizomaLigusticifromdifferentorigins，andscreens5differentialcomponents，whichcanbeusedtoidentifyRhizomaLigusticifromdifferentorigins.KEYWORDSLigusticumsinense;Ligusticumjeholense;Conioselinumvaginatium;Originsidentification;UPLC;Fingerprint;Clusteranalysis;Principalcomponentanalysis;Orthogonalpartialleastsquaresdiscriminantanalysis藁本为伞形科植物藁本LigusticumsinenseOliv.或辽藁本Ligusticumjeholen...