AQP-4、CRP及VEGF在大鼠脑出血后的表达表达与炎症反应的相关性研究贵阳屮医学院第一附属医院神经外科贵州贵阳550001基金项目：贵州省卫生计生委科技棊金，gzwjkj2015-l-059贵州省科技厅联合基金，黔科合LH字［2015］7281贵阳市科技计划项目［项目编号：筑科合同20141001(24>号】贵阳市高层次创新型青年卫生人才棊金项A(2013筑卫科技合同字创9号)【摘要】目的探讨水通道蛋A-4(AQP-4)、C-反应蛋白(CRP)及血管内皮生长因子(VEGF)在大鼠脑出血后的表达与炎症反应的相关性。方法将90只健康人鼠分为实验组72只。对照组18只。实验组72只，采用自体血制作尾状核脑出血模型，观察大鼠术后行为变化，分别在术后6h、24h、48h、3d、7d及14d处死动物。对照组18只，采用相同方法注入等量生理盐水。HE染色观察组织病理变化，EUSA法检测血淸CRP、VEGF表达，免疫组织化学方法检测AQP-4蛋白的表达。结果实验组Longa评分6h及14d高于对照组(P<0.05),24h、48h、3d及7d评分明显高于对照组(P<0.01)。AQP-4、CRP在6h即升高，24h、48h、3d、7d明显升高，VEGF的表达随出血时间的延长而增加，3d明显增高，7d达高峰，对照组：术后CRP、VEGF无明显增高，AQP-4术后3d内升高，7d、14d升高不明显，差异具有显著意义(P<0.01｝。结论脑出血后AQP-4、CRP、VEGF呈现过度表达，其动态变化与炎症反应的相关性具有正相关性。推没三考以不同的方式参与脑出血后损伤修复的炎症反应。StudyonCorrelationofAQP-4,CRPandVEGFExpressionwithInflammatoryResponseinRatsafterIntracranialHemorrhageZhangBo；WuHuajun；ZhongZhijun；etalDepartmentofNeurosurgery,theFirstAffiliatedHospitalofGuiyangCollegeof---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---TraditionalChineseMedicine(Guiyang,Guizhou)550001【Abstract】ObjectiveToinvestigatethecorrelationofaquaporin-4(AQP-4),C-reactiveprotein(CRP)andvascularendothelialgrowthfactor(VEGF)expressionwithinfammatoryresponseinratsafterintracranialhemorrhage(ICH).MethodsAtotalof90healthyratsweredividedintotreatmentgroup(72rats)andcontrolgroup(18rats).Seventy-tworatsinthetreatmentgroupusedtheautologousbloodtomakethecaudatumICHmodelinordertoinvestigatepostoperativebehavioralchangesofrats.Thoseanimalswereexecutedat6h,24h,48h,3dand7d,respectivelyafteroperation.Eighteenratsinthecontrolgroupwereinfusedtheequivalentnormalsaline(NS)usingthesamemethod.PathologicalchangesoftissueswereobservedbyHEstaining;serumCRPandVEGFexpressionwastestedbyELISA;andAQP-4proteinexressionwastestedbyimmunohistochemistry.ResultsLongascoresat6hand14dinthetreatmentgroupwerehigherthanthoseofcontrolgroup(P<0.05),significantlyhigherat24h,48h,3dand7dthanthoseofcontrolgroup(P<0.01).AQP-4andCRPwererisingat6h,particularlysignificantat24h,48h,3dand7d.VEGFexpressionwasincreasedalongwithprolongedbleedingtime(BT)，particularlysignificantat3dandreachedthepeakat7d.PostoperativeCRPandVEGFwerenotsignificantlyincreasedinthecontrolgroup.AQP-4wasincreasedwithin3dafteroperation,notparticularlysignificantat7dand14d.Thedifferencehasstatisticalsignificance(P<0.01).ConclusionAQP-4,CRPandVEGFoverexpressionisshownafterintracranialhemorrhage.Thedynamicchangesandinflammatoryresponsearepositivelycorrelated.Itisspeculatedthatthosethreefactorshaveparticipatedintheinflammatoryresponseofwoundrepairafterintracranialhemorrhageindifferentapproaches.关键词：脑出血；水通道蛋白-4;C-反应蛋白；血管内皮细胞生长因了；炎症反应---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---【中图分类号】R458.3【文献标识码】A【文章编号】1001-5213(2016)09-0344-02欧洲卒中组织（ESO）2014年发布的《自发性脑出血诊疗指南》及美国心脏病协会/美国卒...