EMS创建香蕉抗寒种质及筛选研究许竹叶王安邦李羽佳王笑一魏卿许奕唐粉玲李敬阳摘要利用化学诱变剂甲基磺酸乙酯（EMS）处理“巴西蕉”无菌增殖芽，突变体通过L-羟脯氨酸（HYP）定向筛选，经低温胁迫筛选出抗性较强的突变体。利用形态观察、生理生化指标等手段鉴定突变体。结果表明，EMS诱变处理“巴西蕉”无菌增殖芽适宜的浓度和时间组合为1.5%+3h;经HYP筛选的突变体在低温胁迫后，其叶片脯氨酸含量提高、丙二醛含量降低、过氧化物酶活性和过氧化氢酶活性增强;诱变后的表型突变主要包括叶片颜色、叶片性状和株型的变异。该研究为高效诱变香蕉提供技术参考及后期选育优良品种提供材料。关键词香蕉;甲基磺酸乙酯（EMS）;抗寒突变体;羟脯氨酸（L-HYP）S668.1文献标识码A0517-6611（2021）19-0041-06doi：10.3969/j.issn.0517-6611.2021.19.011开放科学（资源服务）标识码（OSID）：EstablishmentandScreeningofBananaCold-resistantGermplasmbyEMSXUZhu-ye1，2，WANGAn-bang2，LIYu-激a2etal（1.CollegeofHorticulture，HainanUniversity，Haikou，Hainan571101;2.HaikouExperimentalStation，ChineseAcademyofTropicalAgriculturalSciences，Haikou，Hainan571101）AbstractThechemicalmutagensethylmethanesulfonate（EMS）wereusedtotreattheasepticpropagationbudsofBaxi激ao，andthenmutantswereobtainedthroughL-hydroxyproline（L-HYP）directedscreening，andmoreresistantmutantswerescreenedoutthroughlowtemperaturestress.Morphologicalobservation，physiologicalandbiochemicalindicatorswereusedtoidentifymutants.TheresultsshowedthatthesuitableconcentrationandtimecombinationforEMSmutagenesistotreattheasepticproliferationshootsofBaxi激aowas1.5%+3h;afterlowtemperaturestress，themutantsscreenedbyHYPhaveincreasedprolinecontent，decreasedmalondialdehydecontent，increasedperoxidaseandcatalaseactivities;thephenotypicmutationofsurvivingplantsaftermutationmainlyincludedthevariationofleafcolor，leaftraitsandplanttype.Thisresearchprovidestechnicalreferenceforhigh-efficiencymutagenesisofbananasandmaterialsforlaterselectionofelitevarieties.KeywordsBanana;Ethylmethanesulfonate（EMS）;Coldresistancemutant;L-hydroxyproline（HYP）基金项目现代农业产业体系专项资金（CARS-31-02）;海南省科技廳重点研发计划（政法YF2016054）。作者简介许竹叶（1994—），女，海南文昌人，硕士研究生，研究方向：热带园艺与种苗。*通信作者，副研究员，硕士，从事作物遗传育种研究。-全文完-