丙泊酚对糖尿病SD大鼠局部脑缺血再灌注损伤保护作用的研宄孙洋崔岚宫兵(黑龙江省医院150036)【屮图分类号】R972【文献标识码】A【文章编号】1672-5085(2012)16-0075-03【摘要】FI的采用糖妞病Spngue-DawleWSD)大鼠局灶性脑缺血再灌注模型，观察神经功能缺损评分、脑梗死体积、脑组织病理形态改变情况，探讨预防性应用丙泊酚对糖尿病SD大鼠局灶性脑缺血再灌注损伤是否有保护作用。方法50只雄性健康sD大鼠，腹腔注射链脲佐菌素(strepozotocin，STZ)55mg/kg复制糖似病人鼠模型，72h后剪尾尖采血测定血糖，以后每周测血糖，血糖持续&ge;16.7mmol/L为糖尿病大鼠(50只人鼠造模成功36只)。36只糖尿病人鼠，建立高血糖模型后随机分为2组：高血糖组(n=18＞、丙泊酚+高血糖组(简称丙泊酚组n=18)，各组按脑缺血90min再灌注3h(n=6)、6h(n=6)、24h(n=6｝分为3个亚组。采用线栓法制作人脑屮动脉栓塞模型。比较丙泊酚组与高血糖组各再灌注时间点神经功能缺损评分、脑梗死体积和脑组织病理形态的改变。结果相同再灌注时间点，丙泊酚组比高血糖组神经功能缺损程度减轻，P<O.05；相同时间点丙泊酚组较高血糖组梗死体积缩小，其屮再灌注3、6h组间比较P<0.05,再灌注24h组间比较P<O.01。结论预防性应用丙泊酚能减轻高血糖条件不的局灶性脑缺血再灌注损伤，减轻经功能缺损症状，缩小梗死体积。【关键词】丙泊酚糖尿病脑缺血再灌注EffectsandMechanismsofPropofolonFocalCerebralIschemia/ReperfusionInjuryinDiabaticRats【Abstract】ObjectiveApplyingfocalischemia—reperfusionmodelofSDratontheconditionofhyperglycemia,throughobservingthestateofneurologicimpairmentscore,cerebralpathomorphologyandinfarctionvolumeafterfocalischemia-reperfusiondamageinSDratontheconditionofhyperglycemia.Method：1)5---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---0malehealthyrats,weighing180to220grams,makingthediabeticanimalmodelbyintraperitonealinjectionstreptozotocin(producedbysigmaeompany).Iftherandombloodsugarhigherorequal16.7mmol/LWasregardedassuccessofdiabeticmodelthreedayslater,thenraisedtheratsfor6to7weeks(36succesfull).Alltheratsweredivided2groups：hyperglyeemlagroup(n=18)andpropofol+hyperglycemiagroup(namelypropofolgroupn=18).Eachgroupwagdividedinto3subgroupsaccordingtoreperfusion3h(n=6),6h(n=6)，24h(n=6)ofterischemiafor90minutes.Comparethedifferencesofneurologieimpairmentscore,cerebralinfarctionvolumandpathomorphology.Results1-Therearegreatdifferenceoftheneurologicimpairmentscorebetweenthepropofolgroupandhyperglycemiagroup(P<0.05)，Theinfarctionvolumecanheseenatpropofolgroup，andthepeakWagatreperfusion24hours.Compared6hwith3hand24hwith6hinflunarizinegroup,P<O.01.TheinfarctionvolumeintheflunarizinegroupWaglowerthanthatinthehyperglycemiagroup.P<0.01atreperfusion3hoursand6hours.P<0.05atreperfusion24hours.Cerebraltissuepathomorphology：Influnariz—inegroup,theinjuryofcerebraltissuebecameseriouswithtimewentby，butcomparedwithhyperglycemiagroup,thenumberofneuronwhichbecamedegenerationandnecrosisdecreased，vacuolizationandintertissueedemabecamerelieved.ConclusionOntheconditionofhyperglycemia,pretreatmentofpropofolcoulddecreasefocalischemia-reperfusiondamage,relievetheneurologicimpairmentsymptoms；reduceinfarctionvolunlereducetheneurondegeneration，necrosisandtissueedma.【Keywords】PropofolHyperglycemiaCerebralIschemiarepcrfusion糖尿病是缺血性脑血管病的主要危险因素，临床研究发现糖尿病患者脑血管的发病率、致残率及死亡率均比未患糖尿病者高。随着人们饮食结构的变化及社会、工作压力的增加，糖尿病的发病呈上升趋势，因此对糖尿病患者脑损害及脑保---本文来源于网络，仅供参考，勿照抄，如有侵权请联系删除---◁...