生态学报ACTAECOLOG1CAS1N1CADOI:10.5846/stxb201109131337黄玉茜，韩立思,杨劲峰，王月，韩晓日•花生植株和土壤水浸液自毒作用及土壤中自祢物质检测•生态学报,2012,3209):6023-6()32.HuangYQ,llcinLS,YangJE,WangY,IlanXR・Aulotoxicityofaqueousextractsfromplant,soilofpeanutandidenlificalionofautotoxicsubstancesinrhizosphericsoil.AclaEcologicaSinica,2OI2,3209):6023-6032.花生植株和土壤水浸液自毒作用研究及土壤中自毒物质检测黄玉茜I,韩立思2,杨劲峰I,王月-韩晓日宀1-沈阳农业大学土地与环境学院，辽宁生物碳工程技术研究中心，沈阳110866;2.南京农业大学资源环境科学学院，南京210095)摘要:通过盆栽试验及生物测试研究花生植株、土壤水浸液对其种子萌发和幼苗生长的自毒作用，利用单因子方差分析结合多重比较分析各处理组生物活性差异;采用液相色谱法HPIQ对正茬、连作2、4a和6a的花生根际土壤中酚酸类物质进行定性和定量分析。结杲表明：花生植株不同部位及根际土壤水浸液对花生种子浙发和幼苗生长均具有不同程度的抑制作用，且抑制作川具有浓度梯度效应，浓度越高抑制作用越强,16g/10mL水浸液的抑制作用最人。花生生长受自篙物质的影响，此影响作用强度因自毒物质的來源部位不同而存在差异。种子前发以茎水浸液的自毒作用最为明显。但针对幼苗生长的不同测定指标其部位差异不一致，其中以茎水浸液对幼苗株高和叶面积的自毒作用最为明显，根际土壤水浸液对幼苗主根长、单株稣重和单株于重的自毒作用最为明显。在根际土壤水没液中鉴定到4种酚酸物质，分别为对拜基苯甲酸、香草酸、香豆酸和香豆索，其中香草酸和香豆素含量较高且变化规律性明显，在土壤中的含量随连作年限的增加而上升，连作6a后土壤中的含量达到0.289曲/g和0.025ng/g干土。关键词:花生;连作；白毒作用；自毒物质Autotoxicityofaqueousextractsfromplant,soilofpeanutandidentificationofautotoxicsubstancesinrhizosphericsoilHUANGYuqian12,HANLisi2,YANCJinfeng*,WANGYue1,HANXiaori1'*1LiaoningBi^logkalCarbonEngineeringTechnologyResearchCenter,CollegeofhindandEnvironment,ShenyangAgriculluralUnitersUy,Shenyang110866,CM2CollegeResourceonEnvironmentalScienceyl\anjingAgriculturalUniversity,Nanjing21(X)95yChinaAbstract:Autotoxicityisaspecificformofallelopathythatcausesareductioningrowthofindividualsofthesamespeciesviathereleaseofcheniictilsubstancesintothesoilornutrientsolution.AutotoxicitveffectsoftenaremanifestedasreductionJincropyieldanddifficultywithre-establishmentofplantsinfieldsbecauseoflowseedgerminationandpoorseedlinggrowth.Phenolicacidsareimportantautotoxiccompoundsthathindercontinuouscroppingandthustheirphytotoxicityhasbeenstudiedintensively.Bymeansofpotexperiments,theautotoxicityofaqueousextractsfrompeanutplantsandrhizosphericsoilongerininationandgrowthofpeanutseedlingswastested•Thephenolicacidsinpeanutrliizospliericsoil,subjecttorotationalcroppingor,continuouscroppingfor2,4and6years,werequalitativelyandquantitativelyanalyzedwithhigh-jieHonnanceliquidchromatography(4PLC)・Differencesbetweentreatmentswereanalyzedbyone-wayANOV/VandDuncanxsnewmultiplerangelest.Theaqueousextractsfromdifferentplantpartsandrhizosphericsoilinhibitedseed1基金项目：国家现代农业产业技术体系建设专项资金项目fiycytx-19,CARS-14);辽宁省教育厅科学一般项目C2012236)收稿日期：2011-09-13;修订日期:2012-08-202通讯作者Correspondingauthor.E-mail:hanxiaori@163.comhttp://www.ecologica.cngerminationtodifferentdegrees,andshowedaconcentrationgradienteffectontheinhibitionofseedgermination・Inhibitionwasslnnigerwithincreasedextractconcentration,andtheinhibitoryeffectwashighestat16partsaqueousextractto10partsdeionizedwater(6g/10mL).Atthelowestextractconcentrationof4g/10mL,the...