中国油料作物学报Chinesejournalofoilcropsciences花瓣色素定量法对甘蓝型油菜白花性状的质量-数量遗传模型分析田露申匕闫朝选打余青青2,郭世星I,牛应泽“(1•四川农业大学油菜研究中心,教育部作物基因资源与遗传改良重点实验室,四川雅安625014;2•南充市农业科学研究所,四川南充637000)摘要:采用无水乙醇提取油菜花瓣色素•在439nm波长下测定提取液的吸光度,实现对甘蓝型油菜白花性状的量化观察,并应用植物数最性状主基因+多基因混合遗传模型多世代联合分析法,对甘蓝型油菜杂交组合(HW243xHZ21-1)和(HW243x中油821)的P,.P2尼角和B2世代群体进行分析,研究花瓣颜色的遗传特性。结果显示:甘蓝型油菜白花性状表现为数量性状,其遗传符合2对加性-显性-上位性主基因+加性-显性-上位性多基因遗传模型(E-0),以主基因作用为主•多基因的作用相对较小;2对主基囚的加性、显性和上位性效应均具有较大的作用;主基因的遗传力较高;受环境影响较小。在F2群体中主基因的遗传率为95.51%和96.35%,多基因遗传率为3.65%和2.43%;在B,群体中主基因的遗传率为53.00%和49.59%,多基因遗传率分别为39.93%和39.84%;在B?群体中主基因的遗传率为97.38%和95.51%,多基因遗传率分别为2.17%和1・57%。关键词:甘蓝型油菜;白花;花瓣色素;吸光度;主基因+多基因;遗传模型:S565.403文献标识码:A:1007-9084(2010)03-0337-08Analysisofthequalitative一quantitativegeneticmodelsofwhiteflowercolorwithpetalpigmentsquantificationinBrassicanapusL・TIANLu一shen1,2,YANChao-xuan1,YUQing一qing2,GUOShi-xing1,NIUYing-ze1*(1.RapeseedResearchCenter/KeyLaboratoryofCropGeneticResourcesandImprovement,MinistryofEducation,SichuanAgriculturalUniversity,Yaan625014,China\2.NanchongInstituteofAgriculturalSciences,Nanchong637000,China)Abstract:WhiteflowercolorisausefulindicatorinBrassicanapusL.Itwasusedincorrelativeselectionandpuritytest,andwasalsousedasagenetictraitmarker・Inthepresentstudythepetalpigmentsofrapeseedwereextractedandquantifiedbyaspectrophotometerat493nminordertoinvestigatethegeneticmodelofflowercolor.Sixgenerations(Pj,P2,F,,F2,B,andB2)frombothoftwocrossesbetweenwhite-andyellow-floweredrapeseedlines(HW243xIIZ21-1andHW243xZhongyou821)wereanalyzedwithamixedgeneticmodelformajorgenesandpolygenes.Resultsshowedthatwhitecolorinrapeseedwasaquantitativetrait,anditsinheritancefittedtoamixedgeneticmodeloftwomajorgeneswithadditive・dominarH・epistaticeffectspluspolygeneswithadditive・domi-nant-epistaticeffects(E-0model).Amongthemttheadditive,dominantandepistaticeffectsofthetwomajorgeneswereequallyimportant.Theeffectsandheritabilityofmajorgeneswerehighwithlowenvironmentalinfluence,andthatofthepolygeneswerealsorelativelyloTlieestimatedheritabilityratesofthetwomajorgeneswere95.51%and96.35%inF2,53.00%and49.59%inB,,97.38%and95.51%inB2,respectively.Theesti-收稿日期:2010-03-22基金项目:国家863计划项目(2009AA101105);国家科技支撑计划项目;四川省教育厅农作物育种专项(2006LD006);四川省寂十一五"油菜育种攻关项目作者简介:田露申(1976男■四川仪陇人■博士研究生,研究方向为油菜遗传育#,Tel:0835-8286737;E-mail:tianlus@163.com木通讯作者:牛应泽(1955-),男,四川中江人,教授•博士生导师,研究方向为油菜遗传育种,Tel:0835-2882583;E-mail:nyz@sicau.edu.onmatedinheritabilityratesofthepolygeneswere3.65%and2.43%inF2,39.93%and39.84%inB】,2.17%and1.57%inB2,respectively.Keywords:BrassicanapusL・;Whiteflower•Petalpigments;Absorbance;Majorgenepluspoly-gene;Genetic2010年9月2010,32(3):337-344model常见的甘蓝型油菜花色为黄色,也有金黄、桔红、乳白、纯白等其它颜色。花色是有用的指示性状,可用于育种相关选择、纯度鉴定或作为遗传标记等。Sylv切...