离体牙短期应用酪蛋白磷酸多肽钙磷复合体再矿化的实验研究［摘耍］目的：评价短期应用10%酪蛋白磷酸多肽钙磷复合体(complexofcaseinphosphopeptideandamorphouscalciumphosphate,CPP-ACP)(商品名：ToothMousse护牙索)离体牙釉质片再矿化的功效。方法：将正畸需要拔出的离体前磨牙15颗从冠根向一分为二，共30个实验样本，用自凝材料包埋于羯料圈内，根据扫描电镜样甜的要求打磨抛光，抛光后的分釉质面积为3mmX3mmo用数字随机表的方法随机分成4个实验组和1个对照组，每组6个。对照组是川37.5%磷酸酸蚀浸泡牙釉质2min后不用护牙索浸泡，实验组均用37.5%磷酸酸蚀浸泡牙釉质2min后分别用ToothMousse护牙素涂搽牙面30min,置于蒸馆｛水中浸泡。每组浸泡的时间逐步累加，A、B、C、D实验组分别浸泡时间为1、5、10、20ho每组待第2天在同一时间涂搽护牙素及在蒸谓水中浸泡的步骤同第1天，每天更换蒸憾水一次。每组连续作用5次。扫描电镜观察及用能谱检测钙的质量分数，统计分析各组釉柱再矿化与浸泡时间的关系。结果：A、B、C、D实验组釉质表面钙质量分数是(67・38±0・89、67・63±0・56、67.71±0.52.69.11±0.27)和酸蚀组65.74±0.74之间的差异有统计学意义(F二21.568,P二0.0000.05)；D实验组与A、B、C组间差异均有统计学意义(P<0.05)o结论：ToothMousse护牙素体外实验在蒸憾水环境中多次短时间每天20h浸泡离体牙釉质在减少脱矿和促进再矿化方面较实验其余3组作用显著。［关键词］酪蛋白磷酸多肽钙磷复合体；釉质；脱矿；再矿化［中图分类号JR783.5［文献标识码］A［文章编号J1008-6455(2014)17-1441-04Calciumandphosphorusteethfromshort-termapplicationofthecomplexofcaseinphosphopeptideandamorphouscalciumphosphateexperimentalstudyonremineralizationCHENYan,ZHOUYan,LIMing-dong,JIANGRong-Ling,TANGJi-wei,LUQi-fang(DepartmentofStomatology,ThePeople，sHospitalofGuangxi,Nanning530021Guangxi,China)Abstract：ObjectiveToevaluatetheeffectofcomplexofcaseinphosphopeptideandamorphouscalciumphosphateapplicationinenamelremineralizationinvitro.Methods30orthodonticwererandomlydividedinto4experimentalgroupsand1controlgroups,eachgroupof6.Thecontrolgroupwith37.5%phosphoricacidetchingofenamelafter2minimmersionWithouttoothmoussesoaking,experimentalgroupswith37.5%phosphoricacidetchingofenamelaftersoaking2minrespectivelywithToothMoussetoothMousseTuchatooth30min,placedindistilledwaterimmersion.A,B,C,Dgroupsweresoakingfor1,5,10,20h・EachstayforseconddaysatthesametimeTuchatoothmousseandwiththefirstdaythestepsofimmersingindistilledwater・Continuousactioneach5times・Electronmicroscopeandenergyspectrumdetectionbymassfractionofcalciumscan,statisticalanalysisontherelationshipbetweentheenamelremineralizationandimmersiontime・ResultsA,B,C,Dinexperimentalgroup,theenamelsurfacecalciummassfractionis(67.38土0.89,67.63±0.56,67.71±0.52,69.11±0.27)hadsignificantdifferenceandacidetchinggroup65.74±0.74between(F=21.568,P=0.0000.05)；experimentalgroupDandA,B,Cbetweengroupdifferenceswerestatistical1ysignificant(P刘烽等[11]研究观察基牙金属烤瓷全冠预备后实验组表面开窗区涂布护牙素后扫描电镜下的形态改变。扫描电镜下实验组牙面表面痕迹较浅，见密集钙质小团的沉积覆盖才面，対照组基牙表面条纹状痕迹明显，凹凸不平，牙面呈凌乱的疏松无泄形状态。证实含氟酪蛋白磷酸肽-磷酸钙复合物可以提高酸蚀才釉质的耐磨性。本研究结果显示：A、B、C、D实验组釉质表面有大量的矿物质沉积，呈球状、块状覆盖于釉质表面，填补釉质表面局限性的小凹陷；实验D组的釉质表面矿物质的沉积比前3组实验组要致密见，经方差分析(ANOVA)A、B、C、D实验组和酸蚀组间的差异冇统计学意义(F二21.568,P二0.0000.05)；D实验组与A、B、C组间差异均有统计学意义(P〈0・05)。本研究说明ToothMousse护牙素体外实验在蒸憾水环境中多...